Kings University OER

ABSTRACT

 

The antimicrobial effects and the phytochemical constituents of Daniella oliveri was investigated against clinical strains of Staphylococcus aureus, Streptococcus pyogenes, Pseudomonas aeruginosa and Fusarium oxysporum.

The plant was found to be active against the test organisms at concentrations ranging from 50mg/ml to 100mg/ml of the crude extract of the plant. The ethanol extract was more active than the ethyl acetate and the aqueous extracts with Streptococcus pyogenes showing more sensitivity with the highest zone of inhibition of 23 + 1.46 at 100mg/ml concentration. While the lowest zone of inhibition recorded was 10 recorded at 80mg/ml against Pseudomonas aeruginosa. The aqueous ex tract of the plant did not show any activity against Pseudomonas aeruginosa. The phytochemical screening of the plant revealed the plant to contain Saponins, Flavonoids , Tannin, Alkaloids and steroids but Glycosides and Phlobantanin were absent. The antimi crobial screening of antibiotic disc showed the organisms to be sensitive to augmentin, ceftriazone, gentamycin, pefloxacin and ciprofloxacin (Ps.aeruginosa), gentamycin, (Str. Pyogenes) gentamycin, ciprofloxacin ( S. aureus.).The thin layer chromatography showed the ethanol extract to contain more constituents than the extracts of the other solvents.

Keywords: Antimicrobial, phytochemical constituents, daniella, staphylococcus species, pseudomonas species, flavonoid, saponin, tannin, alkaloids, steroids, glycosides

.